Figure 8.
Glucose consumption, lactate production and ATP readjustments in co-culture. Microglia and C6 cells were cultured on beads and as monolayer on Petri dishes, respectively. Cultures were performed either in isolated culture conditions for each cell type or mixed in co-culture. Aliquots of the culture media were collected at different time points from t = 0 to t = 24 h for glucose (A) and lactate (B) quantifications. (A): Glucose concentrations in the medium from isolated microglia(◼), isolated C6 cells (•), microglia/C6 cell co-culture (ᐃ), and Petri dishes without cell (♦). (B): Lactate concentrations in the medium from isolated microglia (◼), isolated C6 cells (•), microglia/C6 cell co-culture (ᐃ), and Petri dishes without cell (♦). ATP quantification (C) was performed from perchloric cell extracts by the luciferin–luciferase method. (◼) and (•): ATP contents in microglia and C6 cells, respectively, when in co-culture. Results, from three independent experiments (mean ± SD), are expressed as percentage of the ATP content for the time point compared to the control (3 nmol/106 cells and 1.3 nmol/106 cells for the microglia and the C6 cells, respectively).