Fig. 1.
Three-dimensional analysis of aortic hematopietic clusters. (A) Confocal image of c-Kit and CD31 expression in the mouse aorta region at E10.5 (35 sp) and (B) E11.5. c-Kit+ clusters (green) are attached to CD31+ endothelial cell layer (magenta). Scale bars: 100 μm. See Movie 1 in the supplementary material for E10.5 and Movie 2 for E11.5 3D images. (C) Representative cluster stained for CD31 and c-Kit expression. CD31 is expressed by both endothelial cells and hematopoietic clusters (magenta, top panel), and c-Kit is expressed by hematopoietic cluster cells (middle panel). Merged fluorescence (bottom panel). Scale bar: 20 μm. (D) Higher magnification image of hematopoietic clusters at 35 sp. Scale bar: 20 μm. VA, vitelline artery.