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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Immunol. 2009 Nov 30;184(1):35–44. doi: 10.4049/jimmunol.0803355

Figure 1. IL-15 influences the maintenance and basal proliferation of 2° memory CD8 T cells.

Figure 1

A) B6 and IL-15-/- mice were primed and boosted with LM-ova as indicated. The number of endogenous splenic Ova/Kb specific CD8 T cells were enumerated by multiplying the percentage of Ova/Kb+ CD8+ cells present by the total number of cells recovered. B) 104 OT-I T cells were adoptively transferred into B6 mice which were then primed and boosted with LM-OVA. At day 5 of boosting, bulk CD8 T cells were transferred into B6 and IL-15-/- hosts and recovery of donor OT-I cells in these secondary hosts determined 23 days later. C) B6 and IL-15-/- animals were adoptively transferred with 104 OT-I T cells and primed/boosted as indicated. The mice were placed on BrdU drinking water for 14 days and then the percentage of OT-I cells showing BrdU incorporation was determined. Statistical differences (p<0.003) were observed between the following pair wise combinations: 1° memory B6 vs IL-15-/-; 2° memory B6 vs IL-15-/- and B6 1° vs. 2°; but not (p>0.05) IL-15-/- 1° vs. 2°. A and C are representative data from at least 3 experiments, while B is representative of 2 experiments.