Figure 1. Decreased levels of MDCs and PDCs in patients with PDAC.
PBMCs isolated from individuals with pancreatic duct adenocarcinoma (PDAC) (1 week pre and 12 weeks post surgery) and healthy age matched volunteers were analyzed for DC levels by flow cytometry. The PBMCs were stained with Linage cocktail, HLA DR, CD11c, and CD123 direct conjugated mabs to distinguish all DC subsets from the rest of the cells. The DC levels were calculated from the total amount of PBMCs and compared between the different groups using nonparametric Wilcoxon signed rank test used for paired data and Mann–Whitney test for calculation of p values. Statistically significant differences between individuals with PDAC and healthy controls are indicated as; * = p<0.05, ** = p<0.005, *** = p<0.001.