FIG. 3.
YFV sfRNA2 is truncated at the 3′ end. (A) Scheme of the YFV 3′ UTR. The two possible orientations of the sfRNAs relative to the YFV 3′ UTR are depicted. The positions of oligonucleotides 1648 and 1296, used to determine the orientations of the sfRNAs, are indicated. Total RNAs were isolated from the indicated YFV-infected cell lines or from mock-infected BHK-21J cells and subsequently analyzed by Northern blotting. (B) Northern blot analysis using oligonucleotide 1648, complementary to YFV-17D nt 10,580 to 10,598. (C) Northern blot analysis using oligonucleotide 1296, complementary to YFV-17D nt 10,830 to 10,862.