FIG. 2.
(A) Replication of an HCoV-HKU1 clinical isolate in HAE. HAE were inoculated with diluted nasal aspirates for the first passage (108 copies/ml) and with apical washes from 96 h postinoculation for the subsequent passage. The bars represent real-time PCR analysis of apical media harvested from HCoV-HKU1-infected HAE at 96 h postinoculation or from the inoculating clinical sample. (B) Replication kinetics of HCoV-HKU1 in HAE. Cultures were inoculated with passage 3 virus (108 copies/ml). Data points represent real-time PCR of apical washes from HCoV-HKU1-infected HAE, harvested at the indicated times postinoculation. Data are presented as HCoV-HKU1 RNA copies/ml and are representative of results from experiments performed in duplicate.