Figure 2. Gradient formation.

(a) Microspheres with the same chemical structure but with different densities were produced from PEG precursors labeled with different fluorescent dyes. The microspheres were suspended in 10% serum and added to a PLL-g-PEG treated Pasteur pipette. (b) The microspheres were initially well mixed. (c) Upon centrifugation, the microspheres separated based on their relative densities.