Figure 7. Tim-4.Ig-coated beads induce signals that rescue T cells from apoptosis and induce Bcl-2.
(a) Tim-4 induced the upregulation of Bcl-2. CD3+ T cells were stimulated for 24 and 48 h with Tim-4.Ig/antiCD3/antiCD28, HuIgG1/antiCD3/antiCD28, or no stimulus. Cell lysates were immunoblotted with anti-Bcl-2 and anti-beta-tubulin. Films were stripped and re-blotted with anti-Bcl-xL/S. Gels were analyzed by using NIH ImageJ software and shown is the expression relative to the untreated samples. (b) CD3+ T cells were cultured as in (a) for 24, 48, and 72 h. T cells were then separated from the magnetic beads, stained with CD4-APC or CD8-APC, Annexin V-FITC, and propidium iodide. Samples were analyzed by flow cytometry.