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. Author manuscript; available in PMC: 2010 Oct 4.
Published in final edited form as: J Immunol. 2008 Apr 1;180(7):4706–4713. doi: 10.4049/jimmunol.180.7.4706

Figure 4. Tim-4 expression enhances CHO cells to induce T cell activation.

Figure 4

(a) CD3+ T cells purified from SJL/J lymph nodes were incubated with mitomycin C-treated CHO or CHO-Tim-4 cells, along with soluble anti-CD3 and anti-CD28. T cell proliferation was measured after 48 h by [3H]-thymidine incorporation in triplicate wells. (b) Anti-Tim-4 mAbs can partially block CHO-Tim-4-induced T cell proliferation. T cells were incubated with CHO-Tim-4 as above, along with anti-Tim-4 mAbs at the concentrations indicated. Proliferation was measured after 48 h by [3H]-thymidine incorporation in triplicate wells.