Skip to main content
. Author manuscript; available in PMC: 2011 Oct 10.
Published in final edited form as: Virology. 2010 Aug 1;406(1):127–137. doi: 10.1016/j.virol.2010.07.002

Figure 5. Disruption of emerin localization in HSV-1-infected HEp-2 cells is sensitive to pUS3-mediated and Rottlerin sensitive kinase phosphorylation events but not BIM I treatment or PKC delta activity.

Figure 5

A) Shown are digital confocal images of optical sections taken near the middle of the nuclei of HEp-2 cells that were uninfected (a–c) or infected with WT HSV-1(F) (d–i) or pUS3 kinase-dead (vRR1204) (j–o) with five PFU/cell for 20 hr and treated with vehicle (a–f and j–l) or Rottlerin (g–i and m–o) beginning at five hpi. Cells were stained with antibodies directed against emerin (left column) or pUL34 (center column). Emerin is represented in red and pUL34 in green. B) Digital image of western blot for emerin in HEp-2 cell nuclear lamina preparations. Cells were mock infected or infected with five PFU/cell of either WT or pUS3 kinase-dead (vRR1204) HSV-1(F). At five hpi, cells began treatment with vehicle (D), BIM I (B), or Rottlerin (R) and were harvested for lamina preparations at 16 hpi. The arrow in lane four indicates the predominant middle species, while the dots in lanes five, six, and seven indicate double or single emerin species. C) Shown are digital confocal images of optical sections taken near the middle of the nuclei of HEp-2 cells that were uninfected (a–c), HSV-1(F) (d–i), or pUS3 kinase-dead (vRR1204) (j–o) infected with five PFU/cell for 20 hr and treated with vehicle (a–f and j–k) or BIM I (g–i, and m–o) beginning at five hpi. Cells were stained with antibodies directed against emerin (left column) or pUL34 (center column). Emerin is represented in green and pUL34 in red. D) Digital confocal images taken near the center of nuclei of EGFP-Emerin HEp-2 B2 cells. Cells were un-transfected (a, b, c) or transfected with PRKCDwt-UL34 Duo (d, e, f) or PRKCDdn-UL34 Duo (g, h, i) for 24 hr. Cells were then infected with pUS3 kinase-dead (vRR1204) at an MOI of 20 for 20 hr. Cells were fixed with formaldehyde and stained with antibodies directed against FLAG-PKC delta (middle column) or pUL34 (right column). Emerin is represented in green, PKC delta in blue, and pUL34 in red.