Figure 6.
Spatial resolution: simultaneous measurement of glycolytic rate in neighboring astrocytes and neurons. Two examples of simultaneous measurement of glycolytic rate in a neuron (n) and an adjacent astrocyte (a) in a dispersed culture are shown. Whereas for example (A), the astrocyte is faster than the neuron, in (B) the opposite is true. The other astrocyte pictured in b also presented a low metabolic rate. Bars represent 25 μm. Image contrast was enhanced to make neuronal processes more visible. (C) Hippocampal slices were infected as described in Materials and Methods. Confocal images show colocalization of the glucose nanosensor (top) and the astrocytic marker GFAP (bottom). Bar represents 10 μm. (D) Slices were superfused with 2 mM glucose and ITM was applied, giving a rate of 6.3 μM/s. (E) Distribution histogram of the rates obtained for 68 cells in 25 slices.