. Author manuscript; available in PMC: 2011 Sep 16.

Published in final edited form as: Cell Host Microbe. 2010 Sep 16;8(3):284–291. doi: 10.1016/j.chom.2010.08.001

Table 1.

G-CSF promotes HCMV spread in engrafted NOD/LtSz-scid/IL2Rg_c^null mice

Treatment	PBMC Pre-G-CSF	PBMC Post-G-CSF	Bone Marrow	Spleen	Liver	Kidney	Lung	SMG	Bladder
Engrafted Mock + G-CSF	0/2	0/2	0/2	0/2	0/2	0/2	0/2	0/2	0/2
Non-Engrafted HCMV + G-CSF	0/2	0/2	0/2	0/2	0/2	0/2	0/2	0/2	0/2
Engrafted HCMV − G-CSF	0/3	NA	3/3	2/3	0/3	1/3	0/3	0/3	0/3
Engrafted HCMV + G-CSF	0/3	3/3	3/3	3/3	3/3	3/3	2/3	1/3	1/3

CD34⁺ HSC-engrafted NOD-scid IL2Rg_c^null mice were mock-infected or HCMV-infected at 4 weeks post-engraftment. At 4 weeks post-infection, engrafted mice were administered G-CSF by osmotic pump for 7 days and sacrificed (Engrafted Mock + G-CSF; Engrafted HCMV + G-CSF). 3 mice did not receive G-CSF treatment and were sacrificed at 5 weeks post-infection (Engrafted HCMV − G-CSF). As a negative control, non-engrafted mice were HCMV infected, administered G-CSF for 7 days at 4 weeks post-infection, and sacrificed (Non-Engrafted HCMV + G-CSF). Peripheral blood mononuclear cells, bone marrow mononuclear cells, and organ tissue was tested for the presence or absence of HCMV genomic DNA by nested PCR. Shown are the ratios of mice testing positive for HCMV DNA out of total mice tested. PBMC Pre-G-CSF and PBMC Post-G-SCF indicate peripheral blood mononuclear cells tested before and after mobilization respectively. NA, not applicable; PBMC, peripheral blood mononuclear cells; SMG, submandibular salivary gland.