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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Cell Neurosci. 2010 Jul 24;45(3):258–266. doi: 10.1016/j.mcn.2010.06.017

Fig 6.

Fig 6

Differentiation of human FOXA2 expressing DA neurons from ES cells requires recombinant WNT1 but not Noggin. (A) Human ES cells were differentiated with or without WNT1 and/or Noggin during the first 14 days of the protocol. (B–E) At DIV49, FOXA2+/TH+/β-tubulin+ cells were observed after exposure to SHH-C24II, FGF8A and RA with (B) or without Noggin (C). FOXA2+/TH+/β-tubulin+ cells were not observed at DIV49 without early exposure to WNT1 (D), or WNT1 and Noggin (E). (F) Cell counts revealed a significantly greater percentage of human FOXA2+ neurons (# p < 0.05 ANOVA) and FOXA2+ DA neurons (δ p < 0.05 ANOVA) after exposure to WNT1 with or without Noggin. Scale bar = 50 µm.