Table I. Protein analysis of purified virus particles.
In-gel digestion of purified viruses was performed, and the peptides were analyzed using LC MS/MS. Virus (A) and host (B) proteins identified in two virus preparations are shown. Homologous proteins are not listed when there is a specific protein identified in the same group. Protein groups are listed when there are no unique peptides identified to infer a specific protein. Numbers in parentheses are the number of unique peptides identified. Statistics collected from Sample 1 and Sample 2 are separated by “/.” When peptides identified can only infer a group of proteins, these entries are labeled with “group” in the “Accession number” column, and other statistics referring to a single protein are labeled with “NA.” For a complete list of proteins identified from Sample 1 or 2, refer to supplemental Table S1, A or B, respectively. sp, Swiss-Prot.
| Protein name | Accession number | Protein probability | Peptides total (unique) | Sequence coverage | Group probability |
|---|---|---|---|---|---|
| % | |||||
| A | |||||
| Nucleoprotein (protein N) (nucleocapsid protein) | sp|P03418|NCAP_HRSVA | 1/1 | 218 (11)/165 (12) | 51.4/51.2 | 1/1 |
| Matrix protein (M) | sp|P03419|MATRX_HRSVA | 1/1 | 239 (12)/192 (12) | 69.5/69.5 | 1/1 |
| Fusion glycoprotein | sp|P03420|FUS_HRSVA | 1/1 | 144 (14)/135 (13) | 39.9/44.4 | 1/1 |
| Phosphoprotein (protein P) | sp|P03421|PHOSP_HRSVA | 1/1 | 97 (6)/48 (6) | 51.9/44 | 1/1 |
| Matrix M2 (envelope-associated 22-kDa protein) | sp|P04545|M21_HRSVA | 1/1 | 90 (9)/94 (7) | 60.8/59.8 | 1/1 |
| Polymerase L | sp|P28887|L_HRSVA | 1/1 | 31 (8)/37 (10) | 7.2/8.8 | 1/1 |
| Major surface glycoprotein G | sp|P03423|GLYC_HRSVA | 1/0.9904 | 5 (1)/1 (1) | 5.4/5.4 | 1/0.9904 |
| B | |||||
| Chaperones | |||||
| Heat shock protein HSP90α | sp|P07900|HS90A_HUMAN/group | 0.9937/NA | 4 (2)/NA | 4.4/NA | 0.9999/1 |
| Heat shock protein HSP90β | sp|P08238|HS90B_HUMAN | 0.9937/1 | 4 (2)/14 (3) | 4.4/14.9 | 0.9999/1 |
| Heat shock cognate 71-kDa protein | Group/sp|P11142|HSP7C_HUMAN | NA/1 | NA/11 (5) | NA/21.1 | 1/1 |
| Heat shock 70-kDa protein 6 | Group/sp|P17066|HSP76_HUMAN | NA/0.9584 | NA/1 (1) | NA/5.1 | 1/1 |
| Cytoskeleton | |||||
| β-Actin-like protein 2 | Group/sp|Q562R1|ACTBL_HUMAN | NA/1 | NA/22 (3) | NA/14.1 | 1/1 |
| Cofilin-1 | sp|P23528|COF1_HUMAN | 1/1 | 23 (3)/7 (2) | 31.3/27.7 | 1/1 |
| Keratin, type II cytoskeletal 1 | sp|P04264|K2C1_HUMAN | 1/1 | 9 (2)/13 (6) | 6.1/16.3 | 1/1 |
| Moesin | sp|P26038|MOES_HUMAN | 0.9979/1 | 5 (1)/39 (5) | 6.4/23.3 | 0.9984/1 |
| Filamin A | sp|P21333|FLNA_HUMAN | 0.9989/1 | 2 (2)/22 (7) | 1/4.2 | 0.9989/1 |
| Tubulin, β | Group/group | NA/NA | NA/NA | NA/NA | 1/1 |
| Tubulin, α-1B | sp|P68363|TBA1B_HUMAN/group | 1/NA | 6 (2)/NA | 11.8/NA | 1/1 |
| Putative tubulin-like protein α-4B | Group/sp|Q9H853|TBA4B_HUMAN | NA/0.9999 | NA/6 (2) | NA/10.8 | 1/1 |
| Energy pathways | |||||
| Alkaline phosphatase | sp|P05187|PPB1_HUMAN | 1/1 | 50 (9)/52 (11) | 35.7/43.7 | 1/1 |
| Intestinal type alkaline phosphatase | sp|P09923|PPBI_HUMAN | 0.9999/1 | 4 (1)/20 (5) | 12.1/24.1 | 0.9999/1 |
| Pyruvate kinase | sp|P14618|KPYM_HUMAN | 0.9997/1 | 3 (1)/22 (7) | 5.8/26.4 | 0.9998/1 |
| Glyceraldehyde-3-phosphate dehydrogenase | sp|P04406|G3P_HUMAN | 0.9996/1 | 6 (2)/9 (2) | 25.9/16.1 | 0.9998/1 |
| Host immune response-related | |||||
| Putative annexin A2-like protein | sp|A6NMY6|AXA2L_HUMAN/group | 1/NA | 25 (6)/NA | 23.3/NA | 1/1 |
| Annexin A1 | sp|P04083|ANXA1_HUMAN | 0.9953/1 | 1 (1)/9 (5) | 13/23.1 | 0.9953/1 |
| Complement decay acceleration factor (CD55) | sp|P08174|DAF_HUMAN | 0.9978/1 | 17 (2)/37 (2) | 13.7/23.9 | 1/1 |
| Ubiquitination | |||||
| Ubiquitin | sp|P62988|UBIQ_HUMAN | 1/1 | 12 (3)/15 (3) | 44.7/56.6 | 1/1 |
| Transcription/translation | |||||
| Elongation factor 1α | Group/group | NA/NA | NA/NA | NA/NA | 1/1 |
| Membrane transport | |||||
| Monocarboxylate transporter 1 | sp|P53985|MOT1_HUMAN | 1/0.9983 | 2 (2)/2 (1) | 7.9/2.8 | 1/0.9983 |
| Monocarboxylate transporter 4 | sp|O15427|MOT4_HUMAN | 0.9953/1 | 4 (1)/6 (3) | 2.4/7.5 | 0.9953/1 |
| 4F2 cell surface antigen heavy chain | sp|P08195|4F2_HUMAN | 1/1 | 13 (5)/13 (5) | 11.7/15.2 | 1/1 |
| Others | |||||
| Brain acid-soluble protein 1 | sp|P80723|BASP1_HUMAN | 1/1 | 18 (6)/30 (7) | 42.3/61.2 | 1/1 |