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. 2010 Jan 18;20(10):2333–2347. doi: 10.1093/cercor/bhp310

Figure 1.

Figure 1.

Expression of 5-HT3A in the adult telencephalon. (A, A′, C) Coronal sections from an adult wild-type mouse showing the distribution of 5-HT3A transcripts. (B) Graph showing the density of both 5-HT3A mRNA-expressing cells from adult wild-type animals (gray bars) and 5-HT3A:GFP+ cells from transgenic 5-HT3A:GFP mice (black bars) in the different layers of the somatosensory cortex. Data are means ± standard error of the mean (SEM). (C,D) Sections from identical stereotaxic levels showing “a similar location of both 5-HT3A mRNA-expressing cells from a wild type” mouse (C) and GFP fluorescent cells from a 5-HT3A:GFP mouse (D). (E) Coronal section of a 5-HT3A:GFP mouse counterstained with Dapi (blue) showing the preferential location of 5-HT3A-expressing cells in supragranular layers. Cortical layers are indicated. (F–J) Expression of 5-HT3A:GFP (green) is restricted to subpopulations of interneurons (red). (F,G) Overlays showing the lack of Parv (F) or SOM (G) expression in GFP+ cells. (H–J) Overlays showing the colocalization of GFP with CR (H), VIP (I), or NPY (J). A1, agranular insular cortex; BA, basolateral amygdaloid nucleus; Cg, cingulate cortex area; Ctx, cerebral cortex; G, gustatory cortex area; Hip, hippocampus; M, motor cortex area; Pir, piriform cortex; S1, somatosensory cortex area; Scale bar: (A–A′) 1 mm; (C,D) 700 μm; (E) 500 μm; and (F–J) 100 μm.