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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Immunol. 2010 May 24;185(1):46–54. doi: 10.4049/jimmunol.1000356

FIGURE 2.

FIGURE 2

Minimal cytokine requirements for the differentiation of IL-9–producing cells. FACS-sorted naive CD4 T cells were stimulated for 7 d with anti-CD3 and anti-CD28 in the presence of various polarizing cytokines, with (A) or without (B) exogenous IL-2. Intracellular cytokine expression was analyzed by flow cytometry. The frequencies of cells expressing IL-9 or IL-17 are represented as mean ± SEM of five different donors. *p < 0.05; **p < 0.01.