Figure 2.
A, Representative pictures (60× objective) showing VWF immunostaining depicting newly formed blood vessels in tumoral sections of Tg APPsw, Tg PS1/APPsw and wild-type littermates. VWF staining reveals higher blood vessel density in glioma tumors from wild-type mice compared with glioma tumors implanted in Tg APPsw and Tg PS1/APPsw mice. B, The histogram represents the percentage area of microscopic fields covered by VWF-immunopositive blood vessels quantified by image analysis. ANOVA revealed a significant main effect of the genotypes on the amount of VWF staining per microscopic area analyzed (p < 0.04). Post hoc analyses show that vascularization is significantly reduced in tumor sections from Tg APPsw (p < 0.03) and Tg PS1/APPsw (p < 0.02) compared with wild-type littermates but no significant difference was observed between Tg PS1/APPsw and Tg APPsw mice (p = 0.759).