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HPLC retinoid analysis. Normal phase of retinoids extracted with hexane from four eyes each (A) isotretinoin and (B) untreated. (A and B Insets) The retinyl ester fraction (2.5–5.0 min) was collected, dried under argon, saponified, and analyzed as above. Peaks: (1) retinyl esters; (2) 11-cis retinal oxime syn; (3) 11-cis retinol; (4) all-trans retinol.
