Figure 15. DNA content analysis of murine tissues by FCM.
Individual DNA content histograms from a representative sample of murine spleen (a), liver (b), cerebellar (c), and frontal cortical (d) nuclei (from the same animal) were stained with propidium iodide and treated with RNaseA prior to FCM. Nuclei were isolated and prepared in a manner similar to the analysis of human nuclei, including the addition of control chicken erythrocyte nuclei (CEN). The color-coded overlay of individual histograms in (a-d) are shown in (e), (red = spleen, blue = liver, orange = cerebellum, and green = cortex). Of note is the relative homogeneity in DNA content from the murine brain compared to the DCV found in the human brain.