Figure 1.
Schematic diagrams (Left) and results of yeast two-hybrid analysis with deletion mutants (Right). Amino acid (aa) numbers at domain junctions are shown above schematics and amino acid residues contained in each deletion construct are indicated in clone name (Lower Left). Minimum critical regions necessary for interaction with binding partners are indicated by shaded bar. (A) Myozenin structure illustrating two putative α-helical domains (A1 and A2) and central glycine-rich domain (Gly). (B) α-Actinin-2 structure showing actin-binding domain (ABD), central repeats (R1–R4), spacer (S), and EF hand domain (EF). (C) γ-Filamin schematic illustrating ABD, repeats (numbered), repeats 19 and 20 interdomain insertion (I), and hinge region (H).