Figure 4.
Position of the light chain domain relative to the catalytic domain (CD) measured by time-resolved fluorescence resonance energy transfer (FRET) within Dictyostelium discoideum S1. (Top) Four structural states resolved by FRET, with structural models constructed as described in Reference 70, by adding the converter domain and lever arm of the indicated PDBs to the D. discoideum CD. The structural states illustrate agreement with the FRET-measured interprobe distances. (Bottom) Table showing the distribution of these structural states within each of the four biochemical states. Fractions for M, M + ATP, and M.ADP.V are calculated from data provided in Reference 70 by normalizing to these three structural states; A.M data and mole fractions are from David Kast (personal communication).