Fig. 8.
Effect of ANG II on podocyte beclin-1 expression. A: equal numbers of CIMPs were incubated in medium containing either buffer or ANG II (10−8–10−6 M) for 24 h. At the end of the incubation period, cell proteins were extracted and Western blots were prepared and probed for beclin-1 and actin. Top: beclin-1 expression by control and experimental cells. Bottom: actin expression by cells treated under same conditions. B: equal numbers of CIMPs were treated with either buffer or ANG II (10−6 M) in the presence or absence of N-acetylcysteine (NAC, 50 μM) for 24 h. Subsequently, cells were harvested and Western blots were prepared and probed for beclin-1 and actin. Top: beclin-1 expression by control (C) and experimental cells. Bottom: actin expression by cells treated under similar conditions.