Fig. 2.
DCAMKL-1 and other putative pancreatic stem/progenitor cell markers. Newborn mice pancreas demonstrates DCAMKL-1 staining (A; arrows) and neurogenin 3 (NGN3; B; arrows) (×600). Immunofluorescence staining for DCAMKL-1 (C; red) and NGN3 (D; green) in the newborn mice pancreas. E–F: colocalization demonstrated in merged image with nuclei stained blue with Hoechst dye (×400). Adult mouse pancreatic tissue serial sections stained with DCAMKL-1 (G), NGN3 (H), and nestin (I) (×200). Immunofluorescence staining of newborn mouse pancreas demonstrated the presence of DCAMKL-1 (J) and nestin (K). L–M: colocalization demonstrated in merged image with nuclei stained blue with Hoechst dye (×400). Insets in J–M are magnified images.