Fig. 3. In vivo p19Arf p53 uncoupling and cellular senescence contribute to cancer suppression in compound mutant mice.
(A) Histopathology and senescence analysis of 11-week-old prostates, stained as indicated for H&E, and senescence associated-β-galactosidase (β-gal) in anterior prostates (AP). (B) Quantification (percentage of cells) of the β-gal staining seen on (A) sections from 11-week-old mice (19.6 ± 5.3% for p19Arf−/−; Ptenpc−/− double mutant mice compared with 17.8 ± 4.3% for Ptenpc−/− mice; P = 0.33>0.05, t-test) . (C) p53 staining on AP sections from 11-week-old mice, arrows denote the positive staining of p53 in nucleus of epithelial cells. (D) Quantification of the p53 staining seen on (C) sections from 11-week-old mice. Senescence and histopathology analysis of 11-week-old prostates. Representative sections from three mice were counted for each genotype. Error bars in (B) and (D) represent S.D. for a representative experiment performed in triplicate.