Table 1.
Charge ratios of protein-DNA complexes at corresponding molar ratios of protein to DNA (The charge ratio was calculated based on the total number of histidine, lysine, arginine, aspartic acid and glutamic acid of the fusion protein and the total number of phosphate groups of the DNA molecule.)
| Molar Ratio | 2 | 11.2 | 28 | 56 | 112 | 280 | 560 | 8600 |
|---|---|---|---|---|---|---|---|---|
| GT-30bp DNA | 0.433 | 2.43 | 6.07 | 12.1 | 24.3 | 60.7 | 121 | - |
| PG-30bp DNA | 0.533 | 2.99 | 7.47 | 14.9 | 29.9 | 74.7 | 149 | - |
| PGT-30bp DNA | 0.800 | 4.48 | 11.2 | 22.4 | 44.8 | 112 | 224 | - |
| GT-293bp DNA | 0.0444 | 0.248 | 0.621 | 1.24 | 2.48 | 6.21 | 12.4 | - |
| PG-293bp DNA | 0.0546 | 0.306 | 0.765 | 1.53 | 3.06 | 7.65 | 15.3 | - |
| PGT-293bp DNA | 0.0819 | 0.459 | 1.15 | 2.29 | 4.59 | 11.5 | 22.9 | - |
| PGT-DsRed plasmid | - | - | - | - | - | - | - | 22.4 |