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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Nat Med. 2010 May 2;16(5):558–564. doi: 10.1038/nm.2142

Figure 2. Role of IFN-γ, TNF-α, and CCL3 in IIC upregulation by memory CD4+ T cells.

Figure 2

(a) Naïve or TH1-polarized memory populations generated from Ifng−/− CD4+ T cells were transferred to unprimed mice then infected with A/PR8. Fold-increase in IFN-γ detected in lung homogenate with transfer of memory versus naïve cells for days 2 and 3 post-infection is shown (n = 5). (b) Memory OT-II cells were transferred to unprimed B6 YETI hosts and lung cells stained for indicated surface markers to identify IFN-γ producing cells (eYFP+) (n = 5). Naïve or TH1-polarized memory OT-II cells were transferred to WT, Ifngr−/−, Tnfrsf1ab−/−, or Ccr5−/− hosts then infected with A/PR8. Level of IIC at 40 hours post-infection (c) and (d) viral titer on day 4 post-infection (n = 5 mice per day). Error bars indicate SD; * P < 0.05, ** P < 0.005, *** P < 0.001 (Students t-test).