FIGURE 1.
Induction of DUSP1/MKP-1 by UV radiation. A, wild-type MEFs were either mock irradiated or exposed to 30 J/m2 UV and then incubated at 37 °C for the indicated times. Cells were then lysed, and proteins analyzed by SDS-PAGE and Western blotting using the indicated antibodies (left). DUSP1/MKP-1 mRNA levels were also determined using quantitative RT-PCR (right). B, wild-type MEFs were exposed to 0, 10, 30, or 100 J/m2 UV and incubated for 1 h at 37 °C. Cells were then lysed, and DUSP1/MKP-1 mRNA levels were determined using quantitative RT-PCR (left). Proteins were also analyzed by SDS-PAGE and Western blotting using the indicated antibodies (right). C, wild-type MEFs were either treated with 300 μm H2O2 (Ox) for 3 h or exposed to 30 J/m2 UV (UV) and incubated for 1 h, before addition of 2 μg/ml of actinomycin D. Cells were then lysed at the indicated times, and DUSP1/MKP-1 mRNA levels were determined using quantitative RT-PCR. Experiments were performed three times, each with triplicate determinations, and mean values with associated errors are shown (mean ± S.E.).