Figure 5.
Moderate c-FLIPL overexpression blocks cell death in HeLa wt cells. (A, top) Prediction of the amount of cell death depending on time and concentration of c-FLIPL after stimulation with 3 µg/ml CD95L in HeLa wt cells. (bottom) Prediction of the amount of cell death depending on the concentration of c-FLIPL after 350 min of stimulation with 3 µg/ml CD95L in HeLa wt cells. (B) HeLa wt cells were transfected with the H2 GFP-IRES–c-FLIPL or an mCherry-encoding control plasmid. 48 h after transfection, LZ-CD95L was added to the cells. The graph shows cell death of GFP+ and mCherry+ cells after addition of 3 µg/ml LZ-CD95L. Mean and SEM of three independent experiments are shown. *, P value < 0.05.
