Figure 4.

Thymus cells from Tace/Mx1 mice release less soluble FLT3L into the culture supernatant than those of Control mice. (A) Total thymus cells were incubated with (+) or without (-) recombinant IL-7 (15 μg/ml) for 72 h. Soluble FLT3L released in the supernatant was analyzed by ELISA. (B) Western blot analysis shows significant decrease, but not complete disappearance, of TACE in the thymus cells from Tace/Mx1 mice.