Skip to main content
. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Clin Cancer Res. 2010 Jul 2;16(16):4217–4225. doi: 10.1158/1078-0432.CCR-10-0777

Fig. 4. Apoptosis induced by ABT-737 and ABT-263 is inhibited in whole blood of CLL patients.

Fig. 4

A, heparinised whole blood from patients with CLL was incubated at 37°C for 4 h with different concentrations of ABT-737 or ABT-263 (n=22). B, whole blood from patients with CLL was incubated for 4, 8, or 12 h without (x-x) or with 0.1 μM (▲-▲), 1 μM (◆-◆), or 10 μM (■-■) of ABT-737 (solid lines) or ABT-263 (dotted lines) (n=7). A-B, after incubation, 20 μl of blood were stained with CD5-FITC/CD19-RPE antibody for 20 min, washed with Annexin-buffer (10 mM Hepes, 150 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1.8 mM CaCl2, pH7.4) and the pellet was resuspended in 500 μl Annexin-buffer + 1 μl Annexin-APC before analysis on FACScalibur with a gate on CD5+CD19+ CLL cells.