Figure 1.
Eosinophil EDN degranulation. Eosinophils from younger (open bars) and older (stippled bars) asthma subjects were cultured for 4 hours at 37°C. A, Eosinophils were either not stimulated, stimulated with fMLP or IL-5. Cells were centrifuged and supernatants analyzed by ELISA for EDN shown as box plots. *p=0.025, by Mann-Whitney rank sum test. B, Total EDN was measured by ELISA from the complete lysis of a known number of eosinophils with Triton X-100 and HCl. Error bars indicate standard error of the mean.