FIG. 1.
Infiltration and maturation of antigen-presenting cells in the lungs of mice after intranasal administration of PIKA. Groups of three mice were given 100 μg of PIKA in a volume of 50 μl or 50 μl of PBS intranasally. One group continued to receive daily dosing of PIKA for two additional days. Lungs were harvested 24 h after the final dose. Single-cell suspensions were prepared, and different cell populations were identified by their differential surface expression of CD11b and CD11c. (A) Lung dendritic cells (lung DC [CD11bint and CD11cint]) expressed as a percentage of total cells in the lungs. The bars and error bars represent the means and standard errors for the group. d1, day 1. (B to D) Lung DC, alveolar macrophages (CD11blo and CD11chi), and interstitial macrophages (CD11bhi and CD11−) were identified, and surface expression of CD86 was determined. Gray, isotype control; blue, PBS control; red, PIKA treated. The diagrams are representative of three individual animals from each group from a single experiment.