Table 1.
Apoptotic cell number in OL-1 cultures treated with IGF-I in presence or absence of β-catenin siRNAs.
| Negative control siRNA | β-Catenin siRNAs |
||
|---|---|---|---|
| 2.5 nM | 5.0 nM | ||
| Control | 50.67 ± 2.16 | 56.54 ± 2.30 | 55.84 ± 1.06 |
| IGF | 10.63 ± 1.03 | 13.88 ± 1.10 (↑31%) | 17.12 ± 0.90 (↑61%)* |
OL-1 cells were transfected with β-catenin siRNAs or a control siRNA for 24 hr, followed by culture with or without IGF-I (100 ng/ml) for an additional 24 hr. The number of PI-labeled apoptotic cells is expressed as a percentage of total cells. Numbers in parentheses indicate percentage changes over IGF-I treated cells transfected with a control siRNA. Values represent mean ± SE from 3–5 samples.
*
P <0.05, compared to IGF-I-treated cells pre-transfected with a control siRNA.