Fig. 5.
a–c Immunohistochemical analysis of tumor tissue for angiogenesis markers. a At the termination of the experiment, tumor tissue sections were stained for VEGF and CD-34 (biomarkers for angiogenesis-related genes). Results demonstrated a significant reduction of both molecules following combination therapy. b VEGF expression was quantified using the Fovea Pro 3.0 imaging program (Fovea Pro 3.0, Reindeer Graphics, Asheville, NC). Eight to twelve images from three to five tumors per treatment group were analyzed for VEGF. c CD-34-based vessel count. Blood vessels were counted in 8–15 individual images from three to four tumors per treatment group as described in the “Materials and methods” section