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Immunogenicity of influenza vaccine coated onto microneedles after drying and storage. Influenza virus-specific antibody response at 1, 2, and 4 weeks after immunization with coated microneedles formulated with (+) or without (−) 15% trehalose after storage for up to 1 month at 25°C. (A) Total influenza virus-specific serum IgG, (B) subtype IgG1, (C) subtype IgG2a, (D) subtype IgG2b and (E) IgG1/IgG2a ratio. (n=6 replicates expressed as average ± SEM).
