Figure 8.
Control and mDPP6 RNAi-treated CG cells show distinctive changes in firing properties as sustained current injections are increased in amplitude. Shown are recordings from control and mDPP6 RNAi-expressing CG cells after 10 d or less in culture. A, Control CG cells require higher current injections to reach threshold and maintain a significant delay before firing the first spike at all current amplitudes. CG cells infected with mDPP6 RNAi fire at lower current injections and the delay to first spike gets dramatically shorter as the amplitude of the current injection increases. B, Summary data show that the time to first spike is dramatically shortened with increasing current injections after suppression of mDPP6. The dotted line at 250 ms indicates the maximum duration of the current injection, and thus values at this level indicate a failure to fire a spike (control, n = 8; mDPP6 RNAi, n = 9). Differences are significant for all current injections above threshold. Error bars indicate SEM.