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Gene expression facilitates response to subsequent pulses. Consecutive-pulse responses were compared for cells treated with (A) 0.1 M NaCl, 16 min. period (i.e., 8 min at 0.1 M followed by 8 min at 0.0 M), (B) 0.2 M NaCl, 32 min. period, (C) 0.35 M NaCl, 45 min. period, and (D) 0.5 M NaCl, 60 min. period. (E–H) Similarly treated cells also exposed to cycloheximide.
