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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: J Immunol. 2010 Jul 2;185(3):1375–1378. doi: 10.4049/jimmunol.0903369

FIGURE 1.

FIGURE 1

CTLA-4–B7 interaction inhibits Th17 differentiation in vitro. A, Purified naive CD4+ T cells from spleens and lymph nodes of CD28−/− or WT mice were cultured in the presence of irradiated T cell-depleted BALB/c splenocytes and anti-CD3 (2 μg/ml) with rhIgG1-Fc (12.5 μg/ml), hCTLA-4-Ig (12.5 μg/ml), hamster IgG (5 μg/ml), or anti–CTLA-4 (5 μg/ml) for 48 h. The production of IL-17, IL-4, and IFN-γ was determined by ELISA. B and C, Naive CD4+ T cells from CD28−/− mice (B) or WT mice (C) were activated with anti-CD3 and irradiated T-depleted splenocytes as feeders under Th17-polarized condition in the presence of hCTLA-4-Ig, rhIgG1-Fc, anti–CTLA-4, or hamster IgG. The values represent mean ± SEM. The data are representative of three independent experiments.