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. Author manuscript; available in PMC: 2011 Jul 23.
Published in final edited form as: Circ Res. 2010 May 20;107(2):271–282. doi: 10.1161/CIRCRESAHA.110.219899

Figure 1. Wnt7 family members activate canonical β-catenin signaling, upregulate type I collagen expression, and promote matrix mineralization in C3H10T1/2 cells.

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Panel A, C3H10T1/2 cells were co-transfected with pcDNA3-Wnt7 expression vectors and LUC reporters as indicated. Both pcDNA-Wnt7a and pcDNA3-Wnt7b upregulated TOPLUC activity vs. vector control. FOPLUC (lacks intact TCF/LEF cognate) was not regulated (n = 6 per condition, replicated > 3 times). Panel B, C3H10T1/2 cells were transduced with SFG vectors expressing either Wnt7a or Wnt7b, and gene expression assessed by RT-qPCR. SFG-Wnt7a and SFG-Wnt7b upregulated Col1A1 and Col1A2 vs. the control virus, SFG-LacZ. *, p < 0.05 vs. SGF-LacZ (n = 3 per condition, replicated twice). Panel C, collagen protein was also increased in monolayers transduced with SFG-Wnt7 vectors (n= 3 per condition, replicated ≥ twice). Panel D, alkaline phosphatase enzyme activity was upregulated by Wnt7a and Wnt7b (n = 4 per condition). Panel E, SFG-Wnt7 transduction also increased calcium deposition, revealed by Alizarin red staining (n = 2 per condition, replicated > 5 times).