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. Author manuscript; available in PMC: 2010 Jul 23.
Published in final edited form as: Nat Immunol. 2009 Jul 13;10(8):831–839. doi: 10.1038/ni.1769

Figure 3. Induced premature stop codon in the Themis gene (E430004N04Rik) in 5AT161 mutant mice.

Figure 3

(a) The critical mapping interval of the 5AT161 mutation with the location of key SNPs and microsatellite markers between B6 and 129 or CBA strains. B6 homozygotes (black squares) and heterozygotes (white squares) are shown for affected and unaffected F2 mice along with the number of recombinants (Rec) observed in affected mice for each genetic marker. (b) Sequence trace histograms of the Tyr-489 mutated codon in wild-type and 5AT161 mice. (c) Amino acid sequence alignment of the region surrounding the conserved cysteine residue in the CABIT domain(s) of multiple Themis protein family members. Highly conserved hydrophobic residues (yellow), conserved cysteine residue(s) (red), and putative coiled-coil motif (underlined) are highlighted. In the case of the two domain proteins like Themis itself, the two CABIT domains are indicated by the prefix 1 or 2. The organism abbreviations are: Ccap : Capitella capitata; Dmel : Drosophila melanogaster; Drer : Danio rerio; Hsap : Homo sapiens; Mmus : Mus musculus; Olat : Oryzias latipes.