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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Immunol Rev. 2010 Jul;236:83–94. doi: 10.1111/j.1600-065X.2010.00900.x

Fig. 2. Homeostasis of naive and memory NK cells.

Fig. 2

Memory NK cells were generated by adoptive transfer of wildtype NK cells (CD45.1+) into Ly49H-deficient mice (CD45.2+) followed by MCMV infection. Memory NK cells were analyzed for BrdU incorporation at 50 days post-infection (top plot). BrdU was given in the drinking water for 7 days, and splenic NK cells were purified and stained for antibodies against NK1.1, CD3, CD45.1, Ly49H, and BrdU. The plot is gated on NK1.1+ CD3− CD45.1+ lymphocytes, and the percentage of Ly49H+ NK cells that have taken up BrdU is shown. As a control, naive NK cells were transferred into Ly49H-deficient mice (without infection) concurrent with BrdU treatment (middle plot). In the bottom plot, wildtype B6 mice (not receiving any transferred cells) were given BrdU and analyzed 7 days later.