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. Author manuscript; available in PMC: 2011 Jul 9.
Published in final edited form as: Circ Res. 2010 May 6;107(1):106–116. doi: 10.1161/CIRCRESAHA.109.214601

Figure 1.

Figure 1

Accumulation of mitoTEMPO in mitochondria and scavenging of O2∸ by mitoTEMPO. (A) ESR spectra of various compartments from BAEC following incubation with mitoTEMPO (1 μM) for 1 hour. Cells were lysed cytoplasmic and mitochondrial fractions isolated by centrifugation. Spectra of the extracellular media, cellular cytoplasm or mitochondria (10 mg weight for each sample) are shown. (B) Spin trapping of O2∸ using the spin trap EMPO and xanthine/xanthine oxidase O2∸ generating system in the absence or presence of mitoTEMPO and mitoTEMPO-H. The efficacy of O2∸ scavenging was derived from dose-dependent decrease of ESR amplitude of EMPO-OOH in the presence of mitoTEMPO (C) or mitoTEMPO-H (D).