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. Author manuscript; available in PMC: 2011 Jun 1.

Published in final edited form as: Alcohol Clin Exp Res. 2010 Apr 5;34(6):997–1005. doi: 10.1111/j.1530-0277.2010.01174.x

Effect of A1 receptor blockade in the wake-promoting BF on ethanol induced sleep.

Panel A: Schematic representation of the anatomical location of histologically identified bilateral microinjection sites. All sites (black circles; N=15) were located between anterio-posterior −0.3 and − 0.6 and are mapped bilaterally onto one coronal brain section (−0.4, adapted from Paxinos and Watson, 2007). Abbreviations: ac = anterior commissure; HDB = Horizontal diagonal band; MCPO = Magnocellularis preoptic; SI = Substantia inominata. For identification of unlabeled structures see Paxinos and Watson (2007).

Panel B: A representative photomicrograph depicting the bilateral lesions caused due to microinjections in the BF (large arrowhead) surrounded by ChAT +ve cholinergic neurons in the BF (small arrows). Abbreviations: ac = anterior commissure.

Panel C: Bilateral microinjections of 50 pmol dose of DPCPX into the BF produced a significant reduction in ethanol induced NREM sleep (* = p<0.5; paired t-test) along with a significant increase in wakefulness (*= p<0.5; paired t-test) as compared to vehicle injections. REM sleep remained unaffected.

Effect of A1 receptor blockade in the wake-promoting BF on ethanol induced sleep.

Panel A: Schematic representation of the anatomical location of histologically identified bilateral microinjection sites. All sites (black circles; N=15) were located between anterio-posterior −0.3 and − 0.6 and are mapped bilaterally onto one coronal brain section (−0.4, adapted from Paxinos and Watson, 2007). Abbreviations: ac = anterior commissure; HDB = Horizontal diagonal band; MCPO = Magnocellularis preoptic; SI = Substantia inominata. For identification of unlabeled structures see Paxinos and Watson (2007).

Panel B: A representative photomicrograph depicting the bilateral lesions caused due to microinjections in the BF (large arrowhead) surrounded by ChAT +ve cholinergic neurons in the BF (small arrows). Abbreviations: ac = anterior commissure.

Panel C: Bilateral microinjections of 50 pmol dose of DPCPX into the BF produced a significant reduction in ethanol induced NREM sleep (* = p<0.5; paired t-test) along with a significant increase in wakefulness (*= p<0.5; paired t-test) as compared to vehicle injections. REM sleep remained unaffected.