Figure 3.
(A) Confocal laser scanning microscopy of Doxo fluorescence in αvβ6-expressing H2009 and αvβ6-negative H460 cells treated with free Doxo, LCP20-MFM and SP20-MFM. The Doxo concentration was kept at 4 μM for all samples and incubation time at 2 h. Cell membrane was stained with Alexa Fluor 350 and pseudocolored with green. Alexa Fluor 350 and Doxo were excited at 352 and 485 nm with emissions at 455 and 595 nm, respectively. (B) Growth inhibition assay of H2009 cells after treatment with free Doxo, LCP20-MFM, SP20-MFM and N-MFM. Relative cell viability (%) was calculated as the percentage of viable cells after treatment over the untreated cell control. IC50 values (Doxo concentration at which the relative cell viability is equal to 50%) were used to compare the different Doxo-containing samples and reported in the text. Standard deviation was obtained from 5 replicates (n = 5).