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Localization of the ClfB-binding site in the tandem repeat region of the αC-domain of Fg using recombinant Aα-chain deletions. Increasing concentrations of recombinant α-chain constructs were immobilized on ELISA plates. Exponential-phase Newman ClfA− cells (1×108 c.f.u.) were added to the wells and adherent bacteria were detected by staining with crystal violet. Error bars represent standard deviations for three replicates. The experiment was performed twice with similar results.
