Table 1.
Comparison of cytotoxic effects of wild-type C. jejuni 81-176 and the isogenic CDT mutant
Semi-confluent T84 cells were grown on chamber slides and inoculated with C. jejuni 81-176 and the CDT mutant at a m.o.i. of 10 and incubated for 30 h. T84 cells inoculated with sterile growth medium served as a negative control. Microscopy and image analysis were performed as outlined in Methods. Results are means±sem for three replicates (three observations per treatment).
| T84 cells per field of view | Average area of nucleus (μm2) | Mitotic cells per field of view | Death foci per field of view | |
|---|---|---|---|---|
| Neg. control | 1144±40</sc> | 3.92±0.23 | 12.7±3.2 | 0±0 |
| 81-176 | 660±49* | 4.69±0.22 | 0.7±0.3* | 5.3±1.5* |
| CDT mutant | 865±75*† | 4.04±0.28 | 7.0±1.0† | 5.6±1.5* |
*P<0.05 vs negative control treatment.
†P<0.05 vs C. jejuni 81-176.