TABLE 2.
Oligonucleotide probes used in this study
| Probe | Targeted gene (serogroup) | Source/GenBank accession no. | Tm (°C)a | Sequence (5′-3′) |
|---|---|---|---|---|
| OA-2443 | wzx (O6) | NC_004431 | 79.86 | CCATGTTGTTCATCTTAAACCTAATGAATGCATTGTGGAA |
| OA-2444 | wzy (O6) | NC_004431 | 79.62 | TCGTAGTGAAGCTATAACGTTTCTTTTAACGGTTACATGT |
| OA-2445 | wzm (O8) | AB010150 | 79.41 | TCACACCCATTGTTTATGTACTGAATTCATTACCTGC |
| OA-2446 | wzt (O8) | AB010150 | 79.73 | GCCGATAAACAAAATCAGTCCATTAAACAAGTTGAGCATA |
| OA-2801 | wzy (O11) | Laboratory stock | 80.91 | CGTTCAAGGTGGCAATTATATATTTCCATTGGTCACACTG |
| OA-2695 | wzy (O11) | Laboratory stock | 79.51 | CAGATGGAGTGTTTATGTATGTTCATTTATGCTAGGGGTA |
| OA-2700 | wzx (O15) | AY647261 | 76.6 | GAGTCATTGGTGTATCGAATTTTGGTGATCTGAGTTTTTC |
| OA-2701 | wzx (O15) | AY647261 | 81.53 | GCAATAAGTCAGGGTGCCAATTACCTACTGCCATTATTAA |
| OA-2452 | wzy (O25) | Laboratory stock | 79.73 | ATCCAGAACTTAACGATGTTAGTAGGCATTGTGATTAGTG |
| OA-2802 | wzx (O25) | Laboratory stock | 80.99 | AAATTAAGCCATGCAAGTAGTTTTACAGCGTCATATGCAG |
| OA-2453 | wzx (O27) | Laboratory stock | 79.55 | TCCTGTGCTATTTATGGGTTAGTTCTGATCAATTAACCTT |
| OA-2454 | wzy (O27) | Laboratory stock | 82.2 | TTGCTCTGTTCATAAAAGGCATTAGCACTTATTATGTCGT |
| OA-2697 | wzx (O78) | Laboratory stock | 79.23 | TCTTTTATCACATTGATTGGTGTTTGTTTTCTCTACCCAA |
| OA-2698 | wzy (O78) | Laboratory stock | 79.03 | TTATGAAAGGCTAACTGTTTACTTCGAATTTTCTCATGCT |
| OA-2666 | wzy (O85) | Laboratory stock | 80.13 | TTTCAGTACGTTAACTTTTGGTTGAGTGATGAACAACGTA |
| OA-2667 | wzy (O85) | Laboratory stock | 79.54 | AGTATTAACTCGTTTAGAAACCTTACAAGCTGGGAATGAT |
| OA-2806 | wzx (O114) | AY573377 | 80.82 | TCATAGGAAAGGATTAGAACATTGCTACAAGTGGTGGATT |
| OA-2809 | wzy (O114) | AY573377 | 79.78 | GGATGGAATGTTAATGGGTTATTTATTTCAGAAGCATGGG |
| OA-2670 | wzy (O115) | Laboratory stock | 79.02 | CAGTTTAGATGTTGTCCGATGGATTAATATAACGCTGTTT |
| OA-2671 | wzy (O115) | Laboratory stock | 80.79 | AGGCAGAAGGATGTTTGCTGTTATTTAATTGTATGCATGT |
| OA-2674 | wzy (O126) | Laboratory stock | 79.22 | ACGTAGTATTCTAATAATCGTGCTAACAATATGTGCGCTA |
| OA-2675 | wzy (O126) | Laboratory stock | 79.59 | TGGCATCTAAAATTATAAGTTCGTTAGGATTAGTGGCGAT |
| OA-2703 | wzx (O128) | AY217096 | 79.21 | GCCCATTGCATTCCTAAATTTGAAATGATTAATGCTATCC |
| OA-2460 | wzy (O128) | AY217096 | 82.02 | GCTAGGTATTTAGCAAATTCAACAGATTTGGCTGACTTTG |
| OA-2707 | wzx (O139) | DQ109552 | 79.35 | GGATTTCAGGGCCAATATTTTATGAGTTTTGTAGCCTTAT |
| OA-2708 | wzy (O139) | DQ109552 | 81.03 | ATGGAACCGTATGTACAATACTTTATAATCATGGGCCTGG |
| OA-2464 | wzy (O148) | DQ167407 | 79.01 | GCAATATTTGATACGTTAAGGGTTTATCTTTTCTCGGGAT |
| OA-2811 | wzy (O148) | DQ167407 | 80.22 | CAATGAGCAATATTTCGTACCATTAAGTGCAACAACCTTG |
| OA-2676 | wzx (O149) | DQ868764 | 79.96 | TATGGTATGCAATTACTGATTCATTAAGATTTGGAGGCGT |
| OA-2677 | wzx (O149) | DQ868764 | 79.89 | CGGTGCAAAGTTAATTCCGCTAACGATAATATGTTGTTTT |
| OA-2466 | wzy (O159) | EU294176 | 79.13 | GTTATAATGACAGTAGATTCAATCCTTTTCTTGGGTTGCA |
| OA-2812 | wzx (O159) | EU294176 | 80.08 | GCACTGAGCTATTTGGGTGTTAATTATTATGGTGTATGGA |
| OA-2680 | wzx (O166) | Laboratory stock | 79.39 | GCATGATGGTTTATTTTAGAGTGGATCGGTATTTTGTTGA |
| OA-2816 | wzy (O166) | Laboratory stock | 79.64 | TAGGAACAATAGTTTCGTTTCGAGATATAAGCGTTGATCG |
| OA-2684 | wzx (O167) | EU296408 | 79.35 | GCCATATATACTTCTGCAAATAAAATATTACAGGCGGCTC |
| OA-2685 | wzx (O167) | EU296408 | 79.2 | ACCGCAGTTGTTAATATCAGTAGTGGCAGTATATATCATT |
| OA-2689 | wzx (O173) | Laboratory stock | 79.31 | AGCTGTACTAATGTCATCATACACGATGGTAATTGGTATT |
| OA-2691 | wzy (O173) | Laboratory stock | 79.58 | TCTTAGAAAAGTTAGAGTTCCACCTCTTTTAGCATTGTGT |
| OA-2467 | rfpB (S. dysenteriae type 1) | S73325 | 79.16 | TGGGAGAAGAAAGTTATAAGTTAGCAAGAGAAAGATTCGA |
| OA-2468 | rfpB (S. dysenteriae type 1) | S73325 | 79.86 | AATTTATTATACTTGGCGCTATAGATAAGGAAAACCCCGG |
| OA-2469 | elt | S60731 | 79.8 | ACACATTAAGAATCACATATCTGACCGAGACCAAAATTGAb |
| OA-2470 | elt | S60731 | 80.16 | GCAAAAGAGAAATGGTTATCATTACATTTAAGAGCGGCGb |
| OA-2471 | estAp | M25607 | 79.74 | TTATCTTTCCCCTCTTTTAGTCAGTCAACTGAATCACTTGb |
| OA-2817 | estAp | M25607 | 76.7 | CGTTTAACTAATCTCAAATATCCGTGAAACAACATGACGG |
| OA-2473 | estAh | AY342059 | 76.5 | GTAGCAATTACTGCTGTGAATTGTGTTGTAATCCTGCTTG |
| OA-2819 | estAh | AY342059 | 78.5 | TTTCACCTTTCGCTCAGGATGCTAAACCAGTAGAGTCTTC |
| OA-2475 | estB | AY028790 | 79.11 | TTCTATTGCTACAAATGCCTATGCATCTACACAATCAAAb |
| OA-2476 | estB | AY028790 | 78.1 | AGGTTTTTTAGGGGTTAGAGATGGTACTGCTGGAGCATGb |
| OA-1993 | 16S rRNA | X80725 | 71.9 | TTGTACACACCGCCCGTCACACCAT |
| wl_4006 | TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT |
a
Tm was predicted using OligoArray software, version 2.0, except for probes OA-2700, OA-2817, OA-2473, OA-2819, and OA-1993, for which Tm was predicted using Primer Premier, version 5.0.
b
Probe used in our previous study (12).