Skip to main content
. Author manuscript; available in PMC: 2010 Jun 4.
Published in final edited form as: Nat Med. 2009 Nov 22;15(12):1377–1382. doi: 10.1038/nm.2062

Figure 4. Staphylococcal LTA induces TRAF1 to inhibit TNF-α.

Figure 4

Figure 4

Figure 4

Figure 4

Figure 4

Figure 4

(a) Western blot of TRAF1 in cultured human keratinocytes stimulated by LTA-SA for various times. The predicted molecular weight of TRAF1 is ca. 52 kDa. GAPDH was used as endogenous control. (b) Quantification of IL-6 and TNF-α of skin extracts from C57BL/6 Traf1-deficient mice and wild-type controls preinjected by PBS or LTA-SA 24 h and 2 h prior to wounding. 2 mm of skin around the wound edges was collected for ELISA. (c) Western blot with antibody raised against the N-terminus of human TRAF1 in keratinocyte extract treated with SE and poly(I:C) for the indicated time periods. The predicted molecular weight of N-TRAF1 is ca. 22 kDa. (d) Western blot showing that caspase 8 inhibitor prevented the cleavage of TRAF1 in cultured human keratinocytes. (e) Quantification of Traf1 mRNA expression in skin of germ-free mice and conventional mice. CV: conventional mice; GF: Germ-free mice. (f) Quantification of Traf1 mRNA expression induced by LTA-SA in skin of wild-type, Tlr2−/− and Myd88−/− mice. * P <0.05, ** P <0.01 and *** P <0.001. n.s. no significance. P-values were determined by using Two-way ANOVA in (b) and (f), and Two-tailed t tests in (e). Data are mean ± SEM, and are representative of two independent experiments with n = 3–7 per group.