TABLE 1.
Bacterial strains and plasmids used
| E. coli strain or plasmid | Genotype | Ref. or source | |
|---|---|---|---|
| Strains | |||
| BW25113 | lacIq, rrnBT14, lacZWJ16, hsdR514, araBADAH33, rhaBADLD78 | (35) | |
| BW25113 | pYdeD | (Enhancing periplasmic l-cystine excreation) | (35) |
| BW25113 | pDE, pCA24N | (l-Cystine overproducer) | (35) |
| JW3832 | BW25113 dsbA::KmR | (35) | |
| JW5182 | BW25113 dsbB::KmR | (35) | |
| JW5250 | BW25113 ydeD::KmR | (35) | |
| JW2663 | BW25113 gshA::KmR | (35) | |
| JW2562 | BW25113 yfiK::KmR | (35) | |
| JW5363 | BW25113 bcr::KmR | (35) | |
| JW1905 | BW25113 fliY::KmR | (Weakening l-cystine uptake) | (35) |
| JW1905 | fliY::KmR, pDES, pCA24N | (Weakening l-cystine uptake) | (35) |
| pCA24N | T5-lacpromoter, pQB2-based, CmR | (36) | |
| pFliY | pCA24N, fliYgene on 0.8 kb DNA fragment | (36) | |
| pDsbA | pCA24N, dsbAgene on 0.6 kb DNA fragment | (36) | |
| pSTV29 | lacpromoter, pACYC184-based, CmR | (8) | |
| pYdeD | pSTV29, ydeDgene on 1.5 kb DNA fragment | (8) | |