Figure 2.
K5 reduces capture, and K3 reduces TCR-driven TEM, of EM CD4+ T cells. A. K5 reduces capture. EM CD4+ T cells captured in flow TEM assay across TNF-treated, TSST-1 preloaded CIITA HDMEC co-transduced with lentivirus encoding GFP (GFP CIITA), K3 Em K3Em CIITA), K5 Em (K5Em CIITA), or not co-transduced (CIITA); mean +/− standard error of the mean (SEM) of one representative experiment of three is shown. K5Em CIITA binds 17% as many cells as controls expressing CIITA only. p<0.001 between K5Em CIITA co-transductants and all other samples, and p>0.05 between all other samples. B. K3 reduces TCR-driven TEM. Mean +/− SEM of EM CD4+ T cell % TEM in flow TEM assay across cells described above. “VB2+” and “VB2−“ refer to those T cells that do or do not bind to TSST-1 on the CIITA HDMEC, respectively. ***, p<0.001 **, p<0.01. One representative experiment of three is shown.